Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
نویسندگان
چکیده
منابع مشابه
Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 case...
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cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. the aim of the present study was to determine the species of cryptosporidium among cattle with diarrhea by a nested pcr-rflp technique at cryptosporidium oocyst wall protein ( cowp ). fecal samples from 158 calves aged 1-20 weeks were collected from 10 d...
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ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2001
ISSN: 1080-6040
DOI: 10.3201/eid0701.010109